“Evaluación de la producción de carotenoides en levaduras nativas aisladas de la ciudad de Cali”

Abstract

In this study, the carotenoid production capacity in native yeasts isolated from the city of Cali was evaluated. 30 yeasts were selected, each of them was seeded in duplicate in MSS (solid pigment inducing medium), they were incubated for 5 days at room temperature (25°C). Pigments were extracted from the biomass obtained for characterization by HPLC. They were identified with molecular techniques and the CS13, P10A and P11A strains were made growth kinetics. The highest yield in pigment extraction was obtained by the CS13 strains with 10.2 µg / mL, the P10A strain with 9.7 µg / mL and the P11A strain with 7.5 µg / mL concentration for β-carotene. The kinetics of growth and pigment production during five days was optimal for the P11A strain, from 48 hours its concentration was 109.62 µg / mL and 1403.10 µg / mL at 120 hours of total carotenoids. In this study, it was found that yeasts isolated from aquatic systems are promising for the production of carotenoid pigments (including βcarotene), making their extraction and characterization viable for future biotechnological studies.