Susceptibilidad a los antimicóticos de levaduras aisladas de sistemas de aguas de la ciudad de Santiago de Cali, Colombia

Abstract

Water systems become a high capacity of microorganisms. The objective of this work was to evaluate the susceptibility to the antifungals of yeasts present in the wastewater and natural waters of the city of Cali. Samples were collected from two types of water: potable (Río Melendez, Puerto Mallarino) and residual (Canal Sur, Planta de Tratamiento Cañaveralejo-PTAR). Yeasts were isolated in sabouraud and CHROMagar Candida in the commercial identification with API 20 C AUX (BioMérieux) and in the sequence analysis of the ITS1- 5.8S-ITS2 region. The minimum inhibitory concentration (MIC) of fluconazole and amphotericin was determined by the microdilution method, and the influence of some physicochemical parameters and heavy metals was correlated with the possibility of yeasts and antifungals. With the phenotypic tests, the 15 yeast species are identified and the molecular biology techniques six species, the morphotype of the property that can belong to the new species of the genus Candida. 100% of the strains were sensitive to Amphotericin B, and 44.3% were sensitive, 19.23% were sensitive dose-dependent (DDS) and the remaining 25% were resistant to fluconazole. Finally, a correlation has been found between the minimum inhibitory concentration, some physicochemical parameters and the concentration of heavy metals on the antifungal sensitivity of yeasts associated with aquatic systems in the city of Cali.